In this study, a terrestrial aquatic DOM sample was utilized for fractionation into various components.
For each condition, 160 μg of protein sample was utilized for the experiment.
Two micrograms of total RNA from each sample was utilized for sequencing library construction.
Since information on patients' asthma severity was unavailable for this sample, a separate asthma sample was utilized for HRQOL disease severity comparisons.
Forty samples were sent for metabolic profiling analysis (n = 20 per group), and another 40 samples were utilized for cell culture and phenotype analysis (n = 20 per group).
As de-identified patient samples were utilized for this study, patient consent was not required.
Of these, 90 YRI I/II (HAPMAPPT03) samples were utilized for genome-wide discovery.
Their blood samples were utilized for ICC as confocal control specimens but not were used for FCM.
Structures deduced for HPRT harboring mutations Lys103Met and Val160Gly identified in our samples were utilized for the analyses.
All patients whose samples were utilized for this study were enrolled in Institutional Review Board approved protocols at the Center for Cancer Research, National Cancer Institute.
