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Three 10 μm sections from each sample were utilized to extract RNA either for total RNA or miRNA analyses.
The average Ct values of all available reference gene assays within a sample were utilized for calculation of ΔCt.
After real time PCR, samples were recovered from capillaries by reverse centrifugation into microcentrifuge tubes (Eppendorf 0.2 μl), and mixed with blue loading buffer and finally 10 μl of each sample were utilized in 1% agarose electrophoresis gel (Invitrogen, Palsley, Scotland, UK) and stained with ethidium bromide.
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For the formulation of recognition algorithm, the set of multi-channel CACCMs (CAMCMs) of per sample is utilized to get a balance between discriminative power and computational efficiency, while multiple marginal CIKSVMs are combined by weighted majority voting.
In this study, a terrestrial aquatic DOM sample was utilized for fractionation into various components.
The findings presented are tentative because a convenience sample was utilized in this study.
For each condition, 160 μg of protein sample was utilized for the experiment.
Two micrograms of total RNA from each sample was utilized for sequencing library construction.
In the melting curve analysis, the double-strand DNA amplicon of each sample was utilized as the amplification control.
A reference sample was utilized, made from equal amounts of RNA from each endometrial sample analyzed (22 caruncular and 22 intercaruncular samples).
Since information on patients' asthma severity was unavailable for this sample, a separate asthma sample was utilized for HRQOL disease severity comparisons.
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