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The threshold cycle (Ct) values of each sample were used in the post-PCR data analysis.
The average RPKM values of three biological replicates in each sample were used for further analysis.
Five microliters of these exosomes sample were used for NanoSight LM10 (NanoSight Ltd) analysis after dilution 1 100 in PBS.
A total of 500,000 cells per sample were used for both immunoprecipitation and control (IgG, SantaCruz).
In the laboratory tests, two identical core plugs from every sample were used for each application.
The alignment coordinates of uniquely aligned reads for each sample were used to independently calculate the read depth of each annotated gene.
Two swatches of each sample were used.
Three technical replicates of each sample were used.
Five specimens per sample were used for each tested parameter.
Four different types of drilling mud sample were used in this study.
For extraction, 200 μL of ruminal content per sample were used.
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