Sentence examples for sample were then from inspiring English sources

OTUs from each sample were then clustered according to dissimilarity within each sample.

Whole cell lysates of each sample were then prepared in 1x Laemmli buffer (Bio-Rad) with 5% β-mercaptoethanol for Western blot analysis.

Microhardness and wear resistance of each treated sample were then tested to evaluate the effects of ω-assisted α phase on the surface properties.

Peptides from each sample were then separated by reversed phase nano-HPLC-Chip technology (Agilent Technologies) online-coupled with a 3D ion trap mass spectrometer (model Esquire 6000, Bruker Daltonics).

Top ten exon junctions with the largest EJEI difference between the 4 mesothelioma and the normal lung sample were then examined for differential expression using Quantitative Real Time PCR (qRT-PCR) in the 5 sequenced samples.

Two microliters of each sample were then mixed 1 2 with loading buffer (2× Laemmli sample buffer with 10% β-mercaptoethanol), denatured at 95°C for 5 min and separated on Criterion TGX 12% polyacrylamide gels (BioRad).

The general recommended procedures applied for pure sample were then performed.

Sample were then oven-dried at 80°C to constant weight for the determination of dry weight.

100 μl of sample were then injected into the HPLC and analyzed with the Water's PICO TAG amino acid analyzer.

50 μL of each sample were then transferred in quartz spectrophotometer cuvettes with 2.95 mL of the potassium-phosphate-EDTA solution.

A total of 1,011 of this sample were then randomly assigned to a treatment group, which we attempted to match to available positions at hundreds of firms.