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The 15 mL tubes, including the initial mineral-free control sample, were subsequently agitated with a benchtop shaker for 24 hrs, removed from the shaker and the pH measured again.
Ratios of target gene quantity to each of the three housekeeping gene quantities for each sample were subsequently calculated.
Twenty uL of each sample were subsequently injected into the mass spectrometer.
In our project, ligation products of each sample were subsequently amplified in two PCRs.
Data from the original and total current GSH levels in each sample were subsequently used to calculate the levels of GSSG present in each sample.
The amplicons from each sample were subsequently subjected to a second PCR using different combinations of barcode primers for each sample.
Similar(49)
The sample was subsequently filtered using 0.2 μm filter.
The sample was subsequently cooled to ambient temperature in the same He stream.
The morphology of the sample was subsequently examined using both SEM and TEM.
Each pore water sample was subsequently filtered through a 0.2 μm pore size syringe filter.
To ensure accuracy of the results, each DNA sample was subsequently re-amplified and sequenced.
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