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Three sections of each sample were stained and digitally recorded by Zeiss Axioplan2 microscope and Zeiss AxioVision software (Micro-optik).

After 24-h incubation, 100 μl aliquots of each sample were stained using the L7007 Bacterial Viability Kit (Molecular Probes, Invitrogen, USA) in the dark for 15 min.

Sperm from each sample were stained by modified Papanicolaou stain and evaluated manually for normal morphology.

As a comparison the sample were stained with isotype matched controls.

Two grids per sample were stained with 4% aqueous uranyl acetate in the dark at room temperature followed by 3 distilled water washes (slightly warmed) followed by 4 minutes in Reynold's lead citrate and 3 more distilled water washes as above.

Ten sarcoid samples and 1 skin sample were stained.

Subsequently, each sample was stained with DAPI or TO-PRO3 and then analyzed by flow cytometry.

c: the first column of each sample was stained with CBB.

Each sample was stained with haematoxylin and eosin.

The sample was stained with 2% (w/v) uranyl acetate.

After complete drying, each sample was stained with SYBR green I nucleic acid gel stain (Invitrogen).