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After washing with ice-cold PBS, the cytoplasmic and nuclear proteins of each sample were separately harvested using an extraction kit (Beyotime, Shanghai, China) to analyse the translocation of NF-κB into the nucleus.
10 g of each sample were separately added to 90 ml of sterile water containing 0.1% peptone water.
The samples with antioxidants and control sample were separately subjected to oxidative stability test in PetroOxy equipment.
The copy numbers of two OATP1B3 mRNA isoforms in each sample were separately determined by qPCR using the isoform-specific primers.
Using the same quantification and calculation methods as described in the legend of Figure 2, the copy numbers of two OATP1B3 mRNA isoforms in each sample were separately determined.
To inactivate non-specific inhibitors, aliquots of each serum sample were separately treated with receptor destroying enzyme (RDE) prior to being tested with a final serum dilution of 1∶10 (starting dilution for the assays).
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To check the reproducibility of the spectrum, sample was separately spotted several times.
Such phenomena will not necessarily occur if every sample is separately prepared from near neutral stock suspensions for example.
To eliminate the resins, each crude sample was separately dissolved in EtOAc and 2 g of silica gel added to the resulting mixture.
For oxidative degradation, FEX sample was separately heated at 80°C for 2 hr using 1 ml of each of 3% and 30%H2O22.
Each sample was separately exposed to array.
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