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The absolute concentration of constituents in a sample were quantified directly from the measured ion intensities using the known reaction time and the theoretical reaction rate constant for the proton transfer reaction [Lindinger et al. 1998].
Protein levels of each sample were quantified using a protein assay kit (Pierce Biotechnology, Rockford, IL).
The residual leukocytes of each sample were quantified in purified erythrocytes.
Specific transcripts from each sample were quantified using the ABI Prism HT7900 sequence detection system (Applied Biosystems).
CCR5 and HIV-1 copies in each sample were quantified by use of the obtained threshold values from the samples and the corresponding standard curve, constructed from multiple measurements of standards.
Relative amounts of immunotoxic gliadin epitopes in any gluten-containing sample were quantified by a competitive enzyme-linked immunoabsorbent assay (ELISA) using the horseradish peroxidase-conjugated G12 monoclonal antibody (Biomedal, Seville, Spain) [21] that is specific for the hexapeptide QPQLPY from the 33-mer peptide of α2-gliadin [19].
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The DNA concentration of each sample was quantified using Qubit dsDNA BR Kit according to the manufacturer's instructions (Thermo Fisher Scientific).
The protein concentration of each saliva sample was quantified using a BCA Protein Assay Kit Thermo Fisher Scientificc, Waltham, MA, USA), using bovine serum albumin as a standard, following the manufacturer's protocols.
Protein in each sample was quantified using the BCA method.
Total DNA in each sample was quantified by OD260.
Each sample was quantified three times and averaged.
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