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The paper strips containing the GCF/PICF sample were kept in a 1.5-ml Eppendorf tube.
All the samples (1 l bottle per each sample) were kept cold until return to the laboratory.
Shaking and quenching of the samples were done immediately, and the centrifuge tubes with the sample were kept in an ice bath immediately.
We filtered data for low gene expression so that only probes expressed (flagged as "present") in at least one sample were kept; probes that did not change between samples, i.e., identified as having an expression value across all samples between median±1.5, were removed.
Embryos (20 per sample) were kept dried at −80°C before resuspension in PBS supplemented with protease inhibitors and nuclease.
Genes that had an RPKM value ≥0.5 in at least one sample were kept for subsequent analysis.
Similar(44)
Ten percent of sample was kept aside as input.
The studied sample is kept in a quartz glass capillary.
The sample was kept at room temperature.
For precipitation, the sample was kept at −80°C.
The supernatant of each sample was kept for further SeqChIP.
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