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Values from the sample were initially scaled up to the population of Western Europe, which it could be argued was the relevant one, since the area supported half the European breeding population of one species, the greenshank (Tringa nebularia (Gunnerus)).
Ten grams of each sample were initially diluted.
Individuals belonging to the main sample were initially contacted by post.
Between 3 and 8 mg of sample were initially washed at room temperature in water (3 × 30 min) and methanol (3 ×3 0 min) to swell and open up the structure.
Accordingly, 0.2 g of each sample were initially destructed in 4.5 ml concentrated HNO3 (69%) and heated for 4 to 5 h at 105°C for further dissolution in a total volume of 10 ml Milli-Q water.
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However, one A. fumigatus sample was initially negative after PCR amplification.
Each study sample was initially tested in a screening assay at 1 25 and 1 75 dilutions.
The temperature of the coal sample was initially 30 °C and programmed to rise 20 °C per hour.
The sample was initially counted for 150 s to establish a low-noise reference count rate.
In experiment 1, water could infiltrate pores of any size because the sample was initially dry.
This trend was reversed when the sample was initially loaded in compression.
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