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The DNA sequences of each sample were individually aligned and clustered using Aligner and Complete Linkage Cluster tools of the RDP.
Reads unmapped for each sample were individually assembled de novo using Velvet v.1.2.10 [ 41] with K-mer 29.
The ds-cDNA and gDNA pairs from each sample were individually hybridized to BeadChips and processed following standard Infinium procedures.
Equal amounts of trypsin-digested peptides from each sample were individually labeled using one of four iTRAQT™ tags, which are specifically incorporated into free amine groups.
The shotgun reads from each sample were individually assembled to generate non-redundant metasequences using the PCAP software with default parameters.
To further evaluate the sensitivity of colon cancer cells to FasR-mediated apoptosis, primarily cultured tumour cells from each resected sample were individually treated with agonistic anti-Fas MoAb CH-11.
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In addition, each sample was individually assembled with MEGAHIT66.
The dilution of each sample is individually prepared based on the drug concentration and the expected impurity content.
Taxonomic classification of each sample was individually conducted using Ribosomal Database Project (RDP) Classifier (version 2.6) with a confidence threshold of 80%.
Each sample was individually analyzed.
Each sample was individually measured in duplicate.
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