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Aliquots of pre-cleaned 50 ug protein sample were further resuspended in 2D-EF loading buffer containing 7 M urea, 2 M thiourea, CHAPS (4%), NDSB 256 (5%), protease cocktail inhibitors (Roche), 10 mM Tris, pH 8.0 and IPG buffer (0.5%) (pH 3 10 NL from GE Healthcare).
and the original sequencing chromatograms of each sample were further checked by eyes.
Moreover, the findings with this large sample were further confirmed serologically.
The matched small RNA reads in each sample were further annotated and categorized.
RT reaction products from the plasma sample were further amplified with Megaplex PreAmp Primers (Primers A v2.1).
The qRT-PCR results from the TIGR4 adherent sample were further analyzed using the RNA-Seq method.
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The sample was further purified using gel filtration chromatography.
Inoculated sample was further stored at 4 °C for 10 days for spoilage study.
The 1 1 sample was further stored for 186 days and remained amorphous under all conditions.
The low-temperature sample is further activated with carbon dioxide to introduce additional micropores.
ThisX-ray diffraction measurements of these sampeak show that the decrease increasespeandishiftsciatowardshigherimprovementemperaturesrphous–crystas inthephasample
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