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After 12 h, 5 ml of each sample were fixed with 4% formaldehyde for 3 h at room temperature and filtered through polycarbonate filters (type GTTP; pore size, 0.2 μm; diameter, 47 mm; Millipore, Eschborn, Germany).
Sample were fixed with 2% PFA and analysed at CM.
One million cells of each sample were fixed with 70% ethanol in DPBS at 4°C.
DNA samples from Nipponbare, as the reference sample, were fixed in all hybridization experiments.
Parts of each sample were fixed in formalin, embedded in paraffin and stored in the Department of Pathology, Qilu Hospital.
Thirty minutes after exposure, WBC suspensions (5 × 10 cells per sample) were fixed with 10% formaldehyde (Fisher Scientific, USA) and incubated for 10 min at room temperature.
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The sample is fixed to a rigid substrate.
A 20-mg sample was fixed in an infrared cell.
Each sample was fixed in formalin, embedded in paraffin, and sectioned at 4 µm.
Half of each sample was fixed for pathological confirmation.
Cultured samples were fixed in 4% paraformaldehyde, frozen and sectioned prior to histological evaluation.
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