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The wide spectra of each sample were first acquired as a general survey: once the elements composing the sample are identified, the detailed region for each element was then acquired at higher resolution for quantitative (peaks areas) and speciation (chemical states) analysis.
Genes with counts less than the average of embedded negative controls (background noise) in that sample were first set to its background.
Since eight samples were analyzed simultaneously by UDPS in each physical field of the Picotiter plate, reads from each individual sample were first identified using the sample-specific sequence tags in the primers (see Table S2).
PBMCs (5×105 per sample) were first incubated with fluorochrome-conjugated antibodies to CD3, CD4 and CD8 at 4°C, in dark, for 30 min. The cells were then washed with PBS containing 0.2% fetal bovine serum (FBS, Invitrogen, Chicago, IL), and permeabilized with 1× fixation/permeabilization solution (BD Biosciences) for 10 min at room temperature.
One hundred micrograms of sample were first electrophoresed on a 7.5 % SDS-polyacrylamide gel and transferred to PVDF membranes.
PCR technical replicates for each biological sample were first averaged before being used in the GLM analysis.
Similar(32)
In this method, the seawater sample was first processed by removing nitrite and ammonium.
The sample was first allocated across 29 provinces, each of which was taken as a universe.
The sample is first excited with a low-incident power density equal to 70 W·cm−2.
Each sample was first sieved in wet conditions using a 3 − φ sieve.
Each sample was first weighed and then placed into a 5 ml test tube.
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