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For British Columbia and Ontario, 200 µL and 250 µL, respectively, of sample were extracted using the NucliSENS easyMag automated extraction system and then treated with Turbo DNase ("post-DNase" treatment).
In the optimized procedure, 4 mL of water sample were extracted using a 10 mL vial and adding 0.4 g NaCl (final NaCl content of 10%).
Two replicates of each sample were extracted and analyzed.
While 1 L samples were collected, different volumes of sample were extracted based on the matrix.
Two grams of sample were extracted with 50 mL of 95% ethanol by sonication for 1 h.
The available heavy metals in soil sample were extracted with AB-DTPA reagent and determined through AAS.
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Each sample was extracted using a buffer containing 0.5 or 1 M NaCl.
Peak information of modified nucleosides for each sample was extracted using Agilent Qualitative Analysis software.
The fermented rice sample was extracted thoroughly with methanol, and the solutions were filtered and evaporated under reduced pressure.
Total RNA from the water yam sample was extracted using the Qiagen® RNeasy Plant Mini Kit (Qiagen, Valencia, CA) as described by the manufacturer.
The data sample is extracted from the IABS 75-97.
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