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Three FeNO reference equations (for males, females, and total sample) were established, using only previously correlated factors in a stepwise linear regression model.
To determine which of these antisense strand-matching microarray features showed expression in the human breast epithelium, the absence or presence calls for all probe sets on both microarray platforms in either the normal luminal epithelial or the malignant breast epithelial sample were established using the MAS5 algorithm.
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The mixtures were incubated at 37°C for 30 min, and then the concentration of infectious virus in each sample was established using plaque assays.
The total protein concentration of each sample was established using the Bradford assay (Protein Assay, Bio-rad, Ivry sur Seine, France) with bovine serum albumin as standard.
Differential gene expression profiles of immunomagnetically purified luminal and myoepithelial cell samples were established using the criterion of differential detection by at least two of the four genome-wide microarray platforms, as used previously when comparing the normal luminal with the malignant sample.
The protein concentration of the samples was established using the protein assay based on the Bradford method from Bio-Rad (Hercules, CA, USA), using bovine serum albumin as a standard.
In both samples, categories were established using annual household income with the income categories varying by study.
All sample location coordinates were established using global positioning system procedures, for mapping purposes, and to relocate sample sites, if necessary.
The effects of these preservatives on the limits of M. agalactiae direct PCR detection were established using samples inoculated with mycoplasma cultures.
Global transcriptional profiles of the three samples at two different phases were established using RNA-Seq.
The intra-day and inter-day accuracy and precision were established using samples at the concentrations of 10.0, 5.0, and 1.0 μg/mL in triplicate.
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