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Protein concentrations for each sample were determined using bicinchoninic acid (BCA) protein assay kit (Thermo Scientific).
The expression levels of the messenger RNAs (mRNAs) in each RNA sample were determined using the Thermal Cycler Dice Real Time System (Takara Bio Inc., Otsu, Japan).
The mean value of the internal frictional angle, cohesiveness and shear deformation modulus of the peat soil sample were determined using a direct shear box apparatus in the laboratory.
Total mRNA concentrations from each sample were determined using a spectrophotometer (NanoDrop 2000c, Thermo Scientific, Wilmington, DE, USA).
The EC and TDS of each sample were determined using a digital conductivity meter at 25 °C.
Calcium (Ca2+): The concentrations of Calcium (Ca2+) in the water sample were determined using EDTA titrimetric method.
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The supernatant was carefully removed, and concentrations of each sample was determined using a bicinchoninic acid (BCA) assay.
Finally, absorbance values were calculated for standards and samples and the concentration of each sample was determined using the standard curve.
The flash point of the sample was determined using Pensky Martens closed cup flash point test.
The lattice constant for the basic sample was determined using Nelson Riley extrapolation method.
Peak area of each sample was determined using the HP 3365 series II ChemStation software.
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