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VSMC from internal mammary arteries obtained from our clinical sample were cultured from explants in RPMI (Life Technologies, Barcelona, Spain) containing 10% foetal calf serum (FCS).
Chondrocytes (106 per sample) were cultured for a further 24 hours in 50 ml tubes in BM.
UT7 cells (2 × 10 cells per sample) were cultured for 72 h in the presence of 2 U ml−1 rHuEPO with or without 20 μℳ ABT-888.
Thirteen viral isolates (8 urine samples, 4 saliva samples and one blood sample) were cultured from symptomatic neonates, ten other isolates (all saliva samples) were cultured from asymptomatic, CMV-infected neonates.
Forty independent bacterial clones for each sample were cultured in selective LB (Luria Bertani) medium and the corresponding recombinant plasmids were extracted (PureLink Quick Plasmid Miniprep Kit, Invitrogen) and sequenced.
Each number in the table represents the total number of colonies of tumor cells obtained when the epithelial cells from one sample were cultured in the presence or absence of serum-activated fibroblasts.
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Each positive sample was cultured.
Each submitted sample was cultured on at least one liquid medium and three solid media.
All samples were cultured in blood culture media and subcultured into Brucella agar medium.
The samples were cultured by conventional culturing as previously reported [ 22].
All samples were cultured under the same standardized culture conditions as described before [16], [66] [68].
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