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Multiple sequence alignments for each sample were constructed with ClustalX.
Lists of transcripts (as Eucalyptus gene orthologs) present in each sample were constructed for comparing galled and ungalled samples (presence/absence analysis).
Clone libraries of 16S rRNA and hzo genes of anammox bacteria in each sample were constructed according to Weidner (1996) for analyzing the community structures.
Statistical analysis was carried out using R. MetaPhlan result files were read into R, and matrices containing the relative abundances of taxa at phylum and genus level for each sample were constructed.
RNA-seq libraries for each sample were constructed from 250 ng total RNA using the polyA selection and transposase-based non-stranded library construction (Tn-RNA-seq) described previously [ 35].
Next, barcoded Illumina sequencing libraries with a different barcode used for each sample were constructed from the fragmented DNA according to the protocol described in Meyer and Kircher [ 21].
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The New York City sample was constructed following comparable steps, based on local polls by The Times and CBS News in 2002 and 2003.
Similarly, the New York City sample was constructed following comparable steps, based on local polls by The Times and CBS News in 2002 and 2003.
The sample was constructed to ensure representation of postdocs, 292 of whom responded.
Each sample is constructed as follows: Each sample contains three users collected from Twitter.com.
This sample was constructed based on the IRAS all-sky survey and the GALEX All-Sky ImaGALEX All-SkyAIS).
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