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Five successive extractions from each soil sample were combined together to obtain enough DNA and to minimize the DNA extraction bias.
PCR products for each sample were combined and purified using a PCR cleanup kit (Invitrogen corp., Carlsbad, CA).
Whenever different channels of the same sample were combined for analysis, the read counts were summed prior to normalization.
The sequences from one or more flow cells (each containing a portion of the sample) were combined to form a set of 350,000 signatures.
The cDNA from one Cyanine-3 (Perkin Elmer, Boston, MA) reaction (reference sample) were combined with those from a Cyanine-5 (Perkin Elmer, Boston, MA) reaction (experiment sample) and were co-hybridized to oligo array.
All three extracts for each sample were combined and lyophilized.
Similar(12)
Additional file 3: Evaluation of protein quantification; The standard sample was combined at a ratio of 0.5 1 2 by volume.
Reflected light from the tissue sample is combined with reflected light from the reference.
Then the wash sample was combined with the main sample.
Following labeling, the sample was combined in desired ratios.
The vectors of the sorted CT values for each sample are combined into a matrix.
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