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Afterwards, the working standards of different concentrations and grape sample were applied using a micro dispenser.
In this paper, GC-TOFMS for the screening of VAPs in microbial sample and GC/CI-MS for quantitative analysis of six VAPs that have the potential to be detected in the sample were applied.
For Cryo-TEM, 4 μl of sample were applied onto a perforated carbon film grid (R1.2/1.3 Quantifoil Micro Tools GmbH, Jena, Germany) and were blotted by filter paper (Whatman 1 μ) for about 3 s.
5 µl drops of each sample were applied to a clean surface and 100 mesh copper grids placed on top of the drops in order to absorb the particles.
Briefly, equal radioactive amounts of each sample were applied to a silica-coated aluminium TLC plate, which was developed in different mixtures of solvents depending on the nature of the TLC.
40 µg of each sample were applied to NuPAGE 4-12% Bis-Tris gel, electrophoresed, proteins stained with Coomassie Blue staining solution, the gel dried and exposed to a PhosphorImager (Molecular Dynamics).
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Each sample was applied in triplicate.
The sample was applied on a 15 20% gradient polyacrylamide gel.
7 uL of sample was applied to the overlayer.
The sample was applied to an RNeasy column centrifuged.
After the sample was applied to the column, unbound proteins were removed by extensive washing.
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