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Feed and fecal sample were analyzed for dry matter, phosphorus, calcium, and magnesium.
All the sample were analyzed using standard procedure (APHA 1995).
Pairs of negative control and each sample were analyzed by t-test.
The concentrations of metals in the effluent sample were analyzed, Table 2.
Aliquots of the supernatant and the PBS sample were analyzed via RP-HPLC.
Five biological replicates of each sample were analyzed.
A minimum of 6000 cells per sample were analyzed.
The sequences produced for each sample were analyzed separately.
Twenty thousand cells from each sample were analyzed and histograms were generated using CellQuest software.
Three different areas of each sample were analyzed to ensure homogenous readings.
Sixteen replicates of each sample were analyzed on three different days (n = 48).
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