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Threshold cycle (Ct) for the gene of interest in both the test samples and control sample were adjusted in relation to a reference gene using the comparative quantification algorithms-ΔCt (Table 1).
Cytokine concentrations in each sample were adjusted by division by the sample urea concentration to reflect dilution during sample collection and handling.
DNA concentrations of each sample were adjusted to 0.1 μg/ml.
Protein concentrations were determined by Bradford assay (Sigma), and levels of each sample were adjusted to ∼500 μg/ml.
The final protein and detergent concentrations in the NMR sample were adjusted to 0.5 and 60 mM [3% (w/v ], respectively.
To make this calculation, aqueous phase analyte concentrations and EEQ of each slurry and sludge sample were adjusted to the total volume of liquid in the raw sample, and solid phase analyte concentrations and EEQ were adjusted to the dry mass of solids in each sample.
Similar(52)
The concentration of each DNA sample was adjusted to 50 100 ng/μl.
The recycle delay for each sample was adjusted after measurement of the T1 with a fast-saturation-recovery sequence.
The sample was adjusted to reflect Japanese demographics in terms of gender, average age, and geographical features.
The pH of the soil sample was adjusted to 3.97, 4.82, 6.07 and 7.04 by CaCO3, separately.
The pH of the NMR sample was adjusted to 4.0.
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