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For each sample, we performed 10 PCR reactions, pooled the products, and loaded the resulting material on a 2% TAE agarose gel.
Using a randomized split of the sample, we performed exploratory factor analysis (EFA) on Group 1 (n = 459) and confirmatory factor analysis (CFA) on Group 2 (n = 459).
For each sample we performed TRPL measurements in the temperature range of 15 300 K in steps of 10 K.
Since FAC-sorting can provide only up to 99% purity of the cell sample we performed in situ hybridization of specific riboprobes to cryosections of the murine nose.
Using the independent 2005 sample, we performed ordinary multiple regression of each immune response on its subset of candidate baseline correlates identified from 2004.
After elution of the hybridized fraction of the sample we performed PCR with primers complementary to the adapters in order to restore double stranded library and to reduce the relative abundance of COT-1 DNA.
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To estimate the value of internal field in the sample, we perform PR measurement.
To demonstrate feasibility in a biological sample, we perform axial scans of excised mouse skin.
For each individual sample, we perform a Chi-squared test for deviation from the uniform distribution and present the associated box-plots in Figure 3.
For the HD samples, we performed a small-pool PCR as described in37 (Supplementary Fig. S7).
To further identify genome-wide H3.3 distribution changes after DAXX inhibition in PTEN-null/GBM-PDX samples, we performed unbiased chromatin immunoprecipitation sequencing (ChIP-seq) (Supplementary Fig. 9a).
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