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Based on the specific DNA-band pattern and the specific DNA sequence obtained in tissue DNA sample, we established the novel genetic DNA markers derived from the DNA fragments of Sox genes.
From each isolated Caenorhabditis sample, we established cultures, which were cryopreserved and stocked in our collections.
In order to characterize AhR activity of the sediment sample, we established AhR-responsive HeLa cell lines (HAhLP).
To ensure a uniform sample, we established an additional criterion, "average length of hospital stay of less than 90 days", as one of the criteria to compute the standard fee for acute care hospitals.
To calculate the number of expressed exons in each sample, we established a cut-off threshold based on the dcpm values at exonic positions on the opposite (anti-sense) strand.
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To reduce sample complexity (number of protein species/sample) and to enrich proteins being of "lower abundance" out of the unfractionated samples, we established a procedure for subfractionating the Plasmodium falciparum proteome.
First, by hierarchically clustering the log-transformed transcript expression levels in the six samples, we established that the same types of tissue from different patients are more similar than different types of tissue from the same patient (Figure 2a).
For control samples, we established fibroblast cultures from the perilesional tissue of cholesteatomatous patients (perilesional-Fs) as well as from normal human skin of the medial external auditory canal (MEAC-Fs) and from non-auricolar skin (NAS-Fs).
For purposive sampling, we established inclusion criteria in consideration of current practice at the outpatient clinic, and gathered demographic and medical backgrounds of research participant candidates to collect data relevant to adherence of patients with gastric cancer.
To investigate the relationship between the reduction in BMI‐1 protein levels and apoptosis induction by PTC‐209 in primary AML samples, we established a triple staining assay for BMI‐1, CD34, and annexin V.
Within each sampling site we established transects parallel to the water's edge, and randomly selected a maximum of 30 adult trees for each mangrove species with a dbh ≥ 10 cm and spaced with a minimum distance of 5 m between sampled trees.
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