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To get the noise values in the ChIP and control data on the same scale, we multiply the values of density in the control by the noise ratio λ noise, where: (4) To calculate the final density profile for the ChIP sample, we apply the following normalization: (5) HMM is used at the final stage for peak calling.
Finally, for each SNP, individual genotypes were omitted if their peak heights were <25% of the average peak height for that genotypic group as measured across the entire sample; we apply this procedure because poor quality samples often exhibit high background noise that SNPlex can mistake as heterozygotes.
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After performing this random re-association for each blood sample, we applied the same criteria for selecting significantly improved probes as was applied to the observed data, and recorded the number of probes that passed the selection criteria.
For this small sample we applied mixed linear models to analyze the two repeated measurements.
To adjust for yearly changes in the sample, we applied the weights provided by the AHRQ [ 15].
As model MIX did not overcome the stratification present in our highly structured sample we applied a two stage approach.
To find the independent predictors of new plaque formation in the totality of the RA sample, we applied binary logistic regression analysis using both enter and backward methods.
For the family-based GWAS sample, we applied the CNV-FBAT methodology (37) to test the raw intensities for association with the phenotype obesity.
To search for gene expression profiles that were associated with specific groups of melanomas but not necessarily related to an individual sample, we applied a module map similar to that described by Segal et al. [ 20].
On each bootstrap sample, we applied three most commonly used automated model selection procedures (forward, backward and stepwise) where all candidate variables are included at the initial stage of the analysis.
To ensure that the same cortical region was identified in each sample, we applied a mitochondrial stain (cytochrome oxidase) to each section to visually identify layer 4 (called the barrel) of the D1 column/whisker.
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