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Briefly, 7 μL of sample was transferred into a 96-well plate.
The collected sample was transferred to a new vial and centrifuged again to pellet the particle-protein complexes.
Once all samples were collected, 50 μl of each sample was transferred to a 96-well plate where 50 μl of LDH reaction solution was added.
Then the sample was transferred to a tubular furnace and reduced at 300 °C for 1 h with the H2 flow rate of 30 mL min−1.
Each prepared sample was transferred into two separate vials.
After weighing, ca. 20 mg of sample was transferred to a centrifuge tube.
The drilled sample was transferred to a pre-cleaned glass and sealed.
Subsequently, the supernatant from each sample was transferred into respective polypropylene tubes.
Finally, the extracted sample was transferred into auto sampler vials and injected into LC-MS/MS.
Then the sample was transferred into desiccators and allowed to cool down to the room temperature.
Then, 50 g of the mixed sample was transferred into the enrichment medium.
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