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For the measurements described here, de-ionized water (or other liquid sample) was supplied to the central channel of the microfluidic chip using an HPLC pump connected to a pulsation dampener.
The sample was supplied with 1 mM ADP·BeFX.
The Malaysian propolis sample was supplied by Econest Enterprise Sdn.
Blood sample was supplied by a normal adult male volunteer with type B blood.
A minimum of 2.5 μg of cDNA per sample was supplied for labelling and hybridization on custom-made melon microarrays.
Following this, each sample was supplied with 50 μl of 50% pre-washed anti-mouse IgG agarose beads and the suspension was agitated in a rotator for 4 h or overnight at +4°C.
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This step is particularly important if a patient sample is supplied to the laboratory directly by a clinician, because it may not be representative of the surgical specimen sent to the histopathologist.
As control for fractionation, aliquots of protein extracts from each sample were supplied for Histone H3 (nuclear fraction) and GAPDH (glyceraldehyde-3 phosphate dehydrogenase; cytoplasmic fraction) specific immunoblot (Blot).
The matrix of perturbations (i.e., genes by samples) was supplied to IDEM as the "methylation" data.
Samples are supplied for each of these.
Samples are supplied for these in html/ops/ffmail.
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