Sentence examples for sample was stimulated from inspiring English sources

After the initial incubation period and 24 hours in media without FCS to synchronize the cells, each sample was stimulated as per their conditions with DMEM/F12 supplemented with 5% FCS.

Add 200 μl/well of 10, 10 or 10 splenocytes with or without antigen 10 μg/ml in RPMI1640 with 10%FCS (each sample was stimulated in triplicate with or without antigen at each dose).

All samples were stimulated within 5 h after blood collection.

Whole blood samples were stimulated (20 min, 37°C) with HSA conjugates or mock-HSA at a final concentration of 1 µg/ml.

Briefly, samples were stimulated in the indicated conditions for 24 hours then washed without removing the CD3/CD28 beads and resuspended in PBS with 2 µM DCFDA.

The samples were stimulated with D-peptide (WKYMVm; 0.1 µM) [27], PMA (50 nM), or opsonized S. aureus (10 bacteria/cell).

Prior to the start of experiments, the samples were stimulated with 0.1 ms pulses at a frequency of 1 Hz until the CAP had stabilized.

As a positive control for CD8+ T cell reactivity, all samples were stimulated with a collection of WNV-specific CD8+ T cell epitopes that was previously found to be frequently recognised in any given patient [10].

Samples were stimulated overnight with TB antigens (ESAT-6/CFP-10 and PPD) and their CD4+ and CD8+ T cells were assessed for production of CD107a, IFN-γ, IL-2 and TNF-α and the complexity of the responses was determined using SPICE and PESTLE software.

Immediately after blood collection in Lithium-heparin tubes, whole blood samples were stimulated in vitro with a pool of 15-mer peptides overlapping by 11 amino acids and covering the CSP antigen in RTS,S (1.25 µg/ml), medium (negative control) or phytohemagglutin A (PHA, positive control), in the presence of anti-CD28 and anti-CD49d antibodies (BD Biosciences, Belgium).

All aliquots of the blood samples were stimulated within four hours of sampling.