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Each sample was smeared onto glass slides.
The powder sample was affixed to the sample holder and the upper surface of the sample was smeared by a glass slide to get a smooth and uniform surface.
For immunofluorescence, 10 µl of sperm sample was smeared on clean, grease-free slides, air-dried, and fixed in PBS/paraformaldehyde 4% for 15 minutes.
Each sample was smeared on a microscopic slide, fixed with 95%% methanol, dried and stained with giemsa stain.
In technique 1, a sample was smeared onto the glass slide using the edge of another glass slide, whereas in technique 2 the particles were squashed between two slides.
For the differential cell count the sample was smeared over glass slides, fixed and stained with Papanicolau stain and examined in a light microscope at 1000× magnification [ 25, 26].
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The samples were smeared on microscope slides and fixated in methanol.
Samples were smeared on a zero-background quartz plate and scanned from 5 to 90° (2θ).
To get numerous rupture fractures, end faces of samples were smeared with petroleum jelly etc.
To detect CD138 (Syndecan-1) and HPV16-L1 colocalinatimmunofluorescenceescence, 10 µl of sperm samples were smeared on clean, grease-free slides, air-dried, and fixed in PBS/paraformaldehyde 4% for 15 minutes.
Blood samples were smeared on glass slides, and inclusions in reticulocytes were visualized by staining with new methylene blue.
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