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Each sample was set up in triplicate and normalized to RPS17 or 18 S expression by the DDCt method.
The prior posterior distribution of the tip date of the undated sample was set between zero (present day) and one million years, following the analyses of reference22.
An aliquot of each sample was set aside as input control, while the remaining portion was subjected to immunoprecipitate with 5mC antibody or normal mouse IgG.
The light spectra (solar simulator with a UVF or solar simulator with a red-pass filter) were measured at the same place at which the sample was set during the disinfection experiment.
The thickness of the sample was set by a polytetrafluoroethylene stripe (10 or 30 μm).
The height of the dielectric sample was set between 5 and 30 cm.
The contact of the sample was set with the environment after evacuation.
Each soil sample was set into a pressure cell with a membrane filter (0.5 μm) set at the sample bottom.
Temperature of the sample was set at 200 (± 2) K with an Oxford Instruments Cryojet system 700 series.
After the sample was set in the machine and heated to 95 °C, the steam was injected into the sample holder at a flow rate of 0.2 L/min.
As described previously, the tip can be simplified as a combination of a cone and a spherical cap, and the distance between the tip apex and the sample was set at 350 nm.
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