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Each sample was represented in triplicate wells (technical replicates).
To do this, the abundance of each OTU per sample was represented in a bipartite network where each link connected an OTU and a sample with a weight corresponding to the number of sequences.
To correct MS response shift during long analysis duration and different sample enrichment factors, data of each sample were normalised, thus ensuring that each sample was represented by a collection of variables to characterise its metabolic pattern before multivariate data analysis.
The expression level of a gene in a given sample was represented as 2−ΔΔCT, where ΔΔCT = [ΔCT sample, infected)] – [ΔCT sample, uninfected)] and ΔCT = [CT sample)] – [CT β-actin)], where β-actin is the housekeeping gene.
This resulted in 20448 (32×639) pathways reflecting the specific gene expression levels in the different samples, i.e. each sample was represented by the expression levels observed in 639 pathways.
These 752 four-fold pooled M2 DNA samples were then subjected to a three-dimensional (3D) pooling strategy, such that each sample was represented once in an X, Y and Z-coordinate pool.
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(c) Phage ELISA assay on 'Yin-Yang' biopanning simulation against crude rUbi was performed in triplicates and the absorbance for each sample were represented in mean (n = 3) (red line) after background normalization.
(c) Phage ELISA assay of biopanning simulation against crude rUbi was performed in triplicates and the absorbance for each sample were represented as mean (n = 3) (red line) after background removal.
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