Exact(3)
Three microliters of the sample was placed on the carbon-coated copper grid, making a thin film of sample on the grid, and an extra sample was removed using the cone of a blotting paper and kept in a grid box sequentially.
Excess sample was removed using filter paper and grids were negatively stained by the addition of 10 μl 2% (v/v) uranyl acetate in water for 20 s.
To enrich the mRNA, rRNA from each total RNA sample was removed using the Ribominus™ Transcriptome Isolation kit for yeast and bacteria (Invitrogen, USA), in accordance with the manufacturer's recommendations.
Similar(57)
Subsequently, teeth and bones were irradiated with UV lamp for 15 min. Next, the external surface of the samples was removed using a sand-blaster to eliminate both soil and exogenous DNA contaminants.
Ribosome RNA of six RNA samples was removed using Ribo-Zero™ Magnetic Kit (Epicentre).
Genomic DNA contamination from the extracted RNA samples was removed using TURBO DNA-free kit (Ambion).
The black materials on the surface of the samples were removed using a drill.
Shown are the running ground truth of the differential effect (black dotted line), the estimate when the designated number of samples is removed using the proposed pair-wise compatibility-based selection (blue line; see Section 2.2), and the estimate when the designated number of samples is removed using repeated random draws based sample selection (red line; see Section 2.4).
Color coordinates (L*a*b* values) useful for the color simulations were obtained using a fiber optic reflectance spectroradiometer model FS3 (ASD Inc., Boulder Co .. Selected samples were removed using a scalpel under high magnification.
Unincorporated dNTPs from the PCR samples were removed using a gel purification kit (Qiagen).
After incubation, the sperm in the dissociated testicular cell samples were removed using a modified two-gradient (35%70%%) Percoll method.
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