These food containers were specifically designed to avoid physical contact and the experiments were conducted in complete darkness to assure that the mouse's interest for the experimental samples was motivated on the basis of olfaction alone.
The use of a procedure based on bootstrap sampling is motivated by the necessity of increasing the stability and potentially reducing the influence of outliers [ 20].
Sample selection was motivated by the wish to minimise participant awareness of being part of the study, and particularly of being video recorded.
This deep genomic sequencing of the Zambia sample (denoted "ZI") was motivated by preliminary data suggesting that this population has maximal genetic diversity among known D. melanogaster populations, along with minimal levels of admixture from non-sub-Saharan ("cosmopolitan") populations (Pool et al. 2012).
The choice of samples used for experiments was motivated by the availability of enough cells from individual patients and also to optimization of protocols, such as lentiviral transduction, on some, but not all, samples.
This corresponded to assigning a concentration to these samples that was half of the lowest concentration measured and was motivated by sampling ambiguity.
The third goal, to create equal experimental sample sizes for each population size, was motivated by the fact that we did not want our results across different population sizes to be confounded by differing sample sizes.
Proteomics data from T. gondii [19] [21], [66] and Plasmodium spp. [10], [12], [67], [68] exhibit little overlap with each other or with the computationally-defined dataset described above, probably because none of these datasets comes close to saturation sampling of the secretome, and each was motivated by different experimental rationales and employed different protocols.
Braden said he was motivated.
