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Then, the dried sample was manually ground with mortar and pestle.
The sample was manually stirred in order to suspend the shale particles in the anionic polyacrylamide sample before starting the experiment.
Each random sample was manually labeled by one medical doctor.
Every sample was manually median-normalized prior to analysis.
When the alveolar plateau on the capnograph was observed, a breath sample was manually drawn from the subject's breath stream into the syringe.
The grip-to-grip length of each sample was manually entered based on the calculation of this length at 0%% strain by the chosen program.
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Three fields per sample were manually selected and analyzed using the Hamilton Thorne IVOS computerized semen analyzer (Beverly, MA), with the calibration parameters defined in Table S3.
The annotations of each sample were manually verified and their profiles were compared to other samples having the same annotations.
These text files for the peptides in a sample were manually combined into a text file in.pkl format representative of the sample.
Areas covered by 3,3′-diaminobezidine precipitate around the cell membranes (HER2) and in the nuclei (pHSF1) of 60 cells in two regions per sample were manually selected.
For identification of OBPs and CSPs on protein level, about 400 antennae per sample were manually separated and homogenized in 200 μl milliQ water containing 0.1% trifluoroacetic acid (Sigma-Aldrich, St . Louis USA) with a tube fitting pestle.
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