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For STORM data acquisition, the sample was illuminated with oblique illumination (not TIRF) for reduced background signal.
The sample was illuminated by means of a diffuse axial illuminator coupled to a Fiber-lite MI-150 (Dolan Jenner, Boxborough, MA, USA) light source with an approximate color temperature of 3,000 K mounted between the flow cell and the camera.
This procedure proved beneficial because the resolution degraded over time when the sample was illuminated with the electron beam.
When the sample was illuminated by a monochromatic light with a small density of 1 mW/cm2, the obtained photosensitivity, defined as the ratio of the photoconductivity σp to the dark conductivity σd, was as large as ∼105.
In Figure 5, the current is plotted against applied voltage for both in darkness and when the sample was illuminated with photons with energies greater than the quantum well band gap.
Then, we are going to obtain a MIRO profile made up of two different MW frequencies, as if the sample was illuminated by two different radiation sources at the same time.
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In epifluorescence illumination the sample is illuminated from above through the objective lens.
Specifically, the sample is illuminated with a periodic illumination pattern with various frequencies of the form: S (x, y ) = S 0 (x, y ) 2 [ 1 + M 0 cos (2 π f x + α ) ] (2 where S0, M0, fx, and α are the source intensity distribution, modulation depth of the periodic pattern, spatial frequency, and spatial phase respectively.
In structured illumination microscopy, the sample is illuminated with a sinusoidal pattern along one of its lateral dimensions.
Second, the sample is illuminated with TIRF, in which the illumination intensity decays exponentially by the z position of the object.
Curious to see if the fossil melanosomes still contained melanin, Peteya and colleagues used Raman spectroscopy, a technique in which a sample is illuminated by a laser beam.
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