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Each soil sample was homogenised and sieved through 2 mm mesh.
The sample was homogenised by several pipetting.
The sample was homogenised using a Q1A shredder (Qiagen® Ltd).
10 50 mg tissue from each sample was homogenised using the TissueLyser tissue disruptor (QIAGEN, Germany) for 2× 30 seconds at 20,000 rpm.
Each sample was homogenised with a FastPrep cell disrupter instrument (Bio101, ThermoSavant, Qbiogene, Carlsbad, CA, USA) for 2 × 40 s at speed 6.0 and then processed according to the manufacturer's guidelines.
Each sample was homogenised on ice, then suspended in 100 μL of a boiling solution containing 100 mmol/L Tris and 4 mmol/L EDTA (pH 7.75), and incubated at 100°C for 2 min followed by centrifugation at 10,000 ×g for 60 sec at 4°C.
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Sample were homogenised by pipetting the cell lysate directly onto a QIAshredder Spin Column (QIagen) placed in a 2 ml collection tube and centrifuged for 2 minutes at 16,300 x g.
Tissue samples were homogenised in ice-cold homogenisation buffer (supplementary materials) using 15 × 1 s sonication pulses.
After transfer to ice-cold RNeasy RLT lysis buffer (Qiagen, Courtaboeuf, France), LT tissue samples were homogenised using a Precellys tissue homogeniser (Bertin Technologie, Montigny-le-Bretonneux, France).
Frozen samples were homogenised using the FastPrep-24 homogeniser system with lysing matrix D (MP Biomedicals, Illkirch Cedex, France) in RLT buffer supplemented with β-mercaptoethanol (RNeasy Mini Kit, Qiagen, Venlo, The Netherlands) at 4 °C.
Samples were homogenised using a T8 Ultra-Turrax homogeniser (IKA Works, Inc., Wilmington, NC, USA).
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