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Each sample was graded by between 390 (53%) and 513 (70%) raters, which leaves around 36% of all sample-rater pairs that were ungraded and that are regarded as missing data.
Post-hoc tests on inflammation score were not performed since only one sample was graded as 2 on our semi-quantitative scale.
Forty per cent (n=18) of the sample was graded as a paramedic and 60% (n=27) as an emergency medical technician.
When fewer than 50% of the tumour cells were stained to the same degree as normal epithelium, the sample was graded as having reduced expression.
Therefore, when 50% or more of the tumour cells in a given specimen were positively stained to the same degree as normal epithelium, the sample was graded as Axin preserved.
Staining intensity was scored as follows: (1) ≤10% of the tumor area stained, 0; (2) 11 25% stained, 1; (3) 26 50% stained, 2; and (4) ≥51% stained, 3. When stained tumor cells made up more than 25% of the tumor, the sample was graded as positive.
Similar(52)
The observations in TPI index of approximately 10%% of the sample were graded after an interval of 1 week to standardize the investigator for a reproducible result.
Three histologic slices of each sample were graded by two experienced pathologists according to Mankin's grading system [ 17, 18, 28].
The RNA for the cryopreserved samples was graded from zero to five based on interpretation of the bioanalyzer profiles.
All samples were graded by a neuropathologist.
Samples were graded as: +/−, negative/background positive for C4BP, ++, positive and +++, strongly positive.
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CEO of Professional Science Editing for Scientists @ prosciediting.com