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Quantitation of DNA in each sample was extrapolated from its respective standard curve.
Cytokine amount in each sample was extrapolated from the standard curves.
The quantity of DNA (ng/ml) present in the sample was extrapolated from the standard curve according to the Ct value.
The number of copies of the gene of interest in each sample was extrapolated from the corresponding standard curve using the indicated software.
The iCycler Software was used to calculate amplification efficiency of each assay and transcript abundance of each unknown sample was extrapolated from corresponding assay standard curves.
After generation of a standard curve with BioRad CFX Manager 3.0 software, the experimental value for each sample was extrapolated from the average Ct value for each triplicate and all values were increased by 1 to facilitate plotting on a log scale.
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Copy numbers of unknown sample were extrapolated from the standard curve that was generated with the extracted DNA prepared from known C. difficile inocula.
Wherever possible, the results of the random sample were extrapolated to all insurants of statutory health insurance funds (i. e., about 90% of the German population).
The PAM and ACTB mRNA copy numbers for each sample were extrapolated from the standard curve, and the PAM mRNA expression levels were expressed as the number of PAM mRNA transcripts per reference ACTB gene.
The titre of HPAIV in the swab samples was extrapolated from Ct-values on basis of calibration experiments using different log10 dilution series of A/duck/Vietnam/TG24-01/05 (H5N1) virus.
The relative fluorescence unit value obtained from samples was extrapolated against a DNA standard curve to determine the DNA amount.
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