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Only one lemur sample was extracted per extraction session to limit possible cross-contamination between specimens.
The sample was extracted by ultrasonic extraction for 50 min, then centrifuged at 5000 r min−1 for 5 min.
The sample was extracted with blank extractions to monitor against contamination.
And each diluted sample was extracted 5 times using the 3 different automated nucleic acid extraction systems.
Each sample was extracted using a buffer containing 0.5 or 1 M NaCl.
Peak information of modified nucleosides for each sample was extracted using Agilent Qualitative Analysis software.
The fermented rice sample was extracted thoroughly with methanol, and the solutions were filtered and evaporated under reduced pressure.
A DNA sample was extracted from PBMCs using a NucleoSpin RNA/DNA Buffer Set (MACHEREY-NAGEL, Germany) according to the manufacturer's protocol.
Total RNA from the water yam sample was extracted using the Qiagen® RNeasy Plant Mini Kit (Qiagen, Valencia, CA) as described by the manufacturer.
The sample was extracted with acetonitrile, defatted with hexane and cleaned-up on a silica SPE cartridge.
Total DNA of the coculture sample was extracted using a previous method (Yang et al. 2013).
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