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The water samples for oxygen isotopic analyses were prepared by conventional H2O CO2 equilibration (Epstein and Mayeda 1953) where 5 mL of each sample was equilibrated with CO2 gas at 25 ± 0.1 °C for 24 h.
After loading, the sample was equilibrated at the specified temperature (from 140 to 220 °C) for 10 min.
The protein sample was equilibrated with 20 mM Tris buffer, pH 7.4, containing 0.1% SDS.
The sample was equilibrated overnight in the calorimeter at the designated refolding temperature, rescanned from 25 to 50°C next morning to determine the extent of refolding, and discarded.
The sample was equilibrated and 10 fold serial dilutions were performed in whole blood.
After removal of the abundant proteins, the serum sample was equilibrated with 50 mM triethylammonium bicarbonate (TEAB) (Sigma, Tokyo, Japan) using spin concentrators (Corning, Tokyo, Japan).
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In the other, simpler method, the rock sample is equilibrated by circulating the solute through the sample.
A thermocouple is placed in the chamber where the sample is equilibrated.
Samples were equilibrated in both cases at a calibrated temperature of 298 K.
We show these parameters are highly dependent on the ambient humidity to which samples are equilibrated.
Common corn, waxy corn and high amylose corn starch samples were equilibrated to a final water activity of 0.15, 0.33, 0.75 or 0.97.
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