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The weighed tissue debridement sample was dissected into small pieces and placed in a 1.5 ml micro-centrifuge tube.
The muscle layer of each sample was dissected, snap-frozen in liquid nitrogen and stored until further processing.
Each sample was dissected from an area of ≥6 mm.
Placental sample was dissected and frozen at −80 °C until use for foetal DNA preparation.
An additional bone sample was dissected from the diaphyseal section, ground as described above, and aliquots used for analysis of hydroxyproline to estimate collagen content [ 36].
The whole sample was dissected into approximately 1 cm long fragments, which were then longitudinally opened by means of a scalpel.
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Caner cells (Ca), cancerous stroma (Str) of the sample were dissected using the LCM technique.
Seeds from 30 to 40 siliques per sample were dissected and DNA was extracted as described (Gehring et al., 2009).
Briefly, approximately 10 g of fresh renal tissue, obtained from human nephrectomy samples, was dissected, and the obtained cortex was minced and then enzymatically digested with 1 mg/ml of type 4 collagenase.
Tissue samples were dissected immediately after delivery (amnion and choriodecidua were separated by blunt dissection), washed in sterile phosphate-buffered saline (PBS), snap-frozen and stored in liquid nitrogen.
Selected samples were dissected and weight of tissue samples recorded and kept frozen at −20 °C until experimental assays.
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