After drug treatment, half of the spheroids (control and treated; 4 for each sample) were disaggregated by trypsin without chelators for fifteen minutes at 37 degrees and re-seeded as single cell suspensions on flat bottom 96 well plates for conventional 2D culture.
The samples were disaggregated to single cell suspensions by shaking prior to use for pigment determination.
Finally, the samples were disaggregated and homogenized before being sieved to 2 mm (soil metal concentration analysis), and then stored at ambient temperature (t = 22°C) for further analysis.
Biopsy specimens from tumor and adjacent normal tissues were collected from two colorectal cancer (CRC) patients (pt 78 and pt 80), samples were disaggregated by enzymatic treatment and the epithelial cells affinity purified.
The number of communities selected in each district along with the actual sample size was disaggregated by sex.
To achieve research aims, extensive data received from high resolution smart meters fitted to a sample of 130 households was disaggregated into end use events (e.g. shower, dishwasher, etc).
Recall that each of the two samples (MST and Control) was disaggregated into low, medium and high performance levels, yielding a total of 6 subgroups (i.e., MST_Low, MST_Medium, MST_High, Control_Low, Control_Medium, and Control_High).
Similarly, the labor supply was disaggregated into nine occupation groups.
The analysis was disaggregated by relevant background variables.
Data were not disaggregated between male identified MSM, and transgenders, but was disaggregated by men reporting transactional sex and those not.
