Sentence examples for sample was chilled from inspiring English sources

After addition of 0.5% TX-100, the sample was chilled on ice for 15 min and then centrifuged at 35,000×g for 20 min. The resultant supernatant and pellet were saved.

The 10-ml blood sample was chilled on ice, centrifuged at 2000 r.p.m. for 10 min at 4°C and stored in 1.5-ml aliquots at −70°C until analysis.

Ethanol (900 μL) was added to the digestion solution, and the sample was chilled at 0 °C for 10 min. The sample solution was centrifuged at 14000 rpm for 10 min at 4 °C.

The denatured sample was chilled on ice, and then incubated with 100 units (exo-) Klenow fragment (NEB, Ipswich, MA) and dNTP mix (6 mM each; Invitrogen, Carlsbad, CA) in Tris, EDTA buffer, for 2 hr at 37°C.

Once obtained, the sample was chilled and rapidly transported to the laboratory.

Samples were chilled on ice and then passed over chilled Sephadex G25 columns equilibrated in 50 mM sodium acetate/0.5 mM EDTA pH 5.3.

All samples were chilled for 4 h at 5 °C and then frozen to −196 °C.

After heat treatment, samples were chilled on ice and residual xylanase activity was determined as described above.

During incubation, samples were mixed for 10 s every 5 min. After addition of 20 mL of ice-cold deionized water, samples were chilled with liquid nitrogen.

The samples were chilled on ice, and the template-primer mix was then supplemented with Reaction Buffer (50 mM Tris/HCl, 30 mM KCl, 8 mM MgCl2, pH 8.5), Protector RNase Inhibitor (20U), dNTPs (1 mM each) and finally AMV Reverse Transcriptase (10U; Roche, Carnaxide, Portugal), in a 20 µl final volume.

Reverse transcription of mRNA was carried out as follows: 0.5 µg of oligo dT primer in 1 µl volume were added to 11 µl of water containing 2 µg of total RNA and incubated at 70°C for 5 min. Samples were chilled on ice for 3 min, and 8 µl RT mix (4 µl 5x RT-buffer, 2 µl 10 mM dNTPs, 1 µl RNasin Plus RNAase inhibitor and 1 µl M-MLV reverse transcriptase; all components from Promega) were added.