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For each group, each APD sample was assembled on an aluminum plate, with a PCB attached from the back (see Figure 1).
This sample was assembled by empanelling two groups of students of essential analytic interest: (1) a sample chosen randomly from the 6,650 Harvard undergraduates (N = 319), and (2) a "friends" sample (N = 425) composed of individuals who were named as a friend at least once by a member of this random sample (see Supporting Information Text S1 for more details).
Each sample was assembled in ABySS using every 5th kmer ranging from 26bp to 96bp.
Each sample was assembled de novo using MetaVelvet [ 70] and a kmer of 51.
The study sample was assembled from lists provided by The Human Resources Departments.
Between 2.22 and 7.35 Gbp of trimmed data from each sample was assembled independently.
Similar(51)
Then, the high-quality reads of each DNA sample were assembled by the SOAP de novo assembler [ 31] to contig level.
The reads for each sample were assembled into contigs using a reference assembly approach to map reads to the Calamaria probe regions and a de-novo assembly approach to extend the assembly into the flanks (java scripts available upon request from A. Lemmon).
Trimmed reads from each sample were assembled de novo by using the MIRA assembly program (20 ), with a criterion of >95% identity over 35 bp.
Two electrodes with identical sample were assembled with a cotton cloth separator in an electrolytic solution of aq. 1 M H2SO4 solution.
The insulin-EMMIPs obtained by recognizing insulin from sample were assembled onto a magnetic glassy carbon electrode (MGCE) surface in order to form the magnetically controlled membrane under the magnetic field induction, and the membrane was peeled off from the electrode by removal of the magnet after electrochemical measurement.
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