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Each tumour sample was analysed in triplicates.
Each sample was analysed in quadruplicate.
Each sample was analysed in triplicate for each gene study.
The experiments were run in triplicate and each sample was analysed in duplicate.
In addition, the micro structure of the sample was analysed using a scanning electron microscope (SEM).
A volume of 5 μl of each DNA sample was analysed by electrophoresis on a DNA sequencing gel run at a constant 75 W for 1 h.
Initial sample was analysed by HJ,YD.
One additional sample was analysed within the defined sampling periods.
Each sample was analysed by qPCR in triplicate.
Each sample was analysed at least in duplicate.
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Every sub-sample was analysed in triplicate according to procedure described above.
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