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The sample was allocated across strata using disproportional allocation methodology [ 10], which increased the sample size for small strata and decreased the sample size for large strata.
The sample was allocated proportionally for randomly selected kebeles of the district.
Upon collection, each sample was allocated a unique numerical code and the date, time of sample collection, bolus measurements (top diameter, bottom diameter and length), location (GPS co-ordinates and name of road nearest to sample), and the identification of the elephant known to have defecated were recorded.
If the sample was allocated for ICSI, the cells were washed in Cook Gamete Buffer.
The total sample was allocated proportionally to each district based on the number of reproductive age women it has.
The sample was allocated proportionally to the size of the population within each geographic stratum of each city.
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For given K central points (the mean points of the cluster samples), each sample is allocated in a cluster represented by the nearest mean point of its Euclidean distance, and the samples are divided into K clusters.
Significant levels were calculated via a permutation scheme of 5000 replicates where the total sample is allocated at random to groups.
The sample is allocated to provinces and strata within provinces in a way that best meets the need for reliable estimates at various geographic levels.
At family-level classification, the Clostridiales sequences in the HP diet sample were allocated mainly to Lachnospiraceae (57 % of Clostridiales clones), Peptostreptococcaceae (35%% of Clostridiales clones) and Ruminococcaceae (6 % of Clostridiales clones).
Half of the patients in our overall sample were allocated to receive placebo acupuncture intervention (Group P, n = 40), and the other half received active electroacupuncture intervention (Group A, n = 40).
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