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PBS was added into the isolated nuclei and about 3.5 μL sample was absorbed onto a molybdenum grid and dropped into liquid ethane for rapid freezing.
Five µl of each sample was absorbed to a formvar/carbon-coated grid for 1 minute and excess liquid was blotted off using Whatman #1 filter paper.
After the sample was absorbed by the cartridge, the cartridge was washed with 4 mL water.
In addition, very little blood was collected with the automatic biopsy needle and the blood at the surface of the sample was absorbed before freezing the biopsy into nitrogen.
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The resulting record, or spectrum, is ordinarily a straight line indicating that the sample is absorbing no energy broken by peaks at the resonance frequencies.
Blood samples were absorbed onto Whatman FTA Elute absorbent filter paper in Grande Comore, Moheli, and Anjouan islands, on Whatman 903 Protein Saver filter paper (Whatman Inc., Florham Park, NJ, USA) in Mayotte, and collected into Vacutainer tubes (Becton Dickinson, Le Pont-De-Claix, France) in Marseille.
In this technological design, calibration solutions and samples were absorbed into textiles while the potentiometric cells (ion-selective electrodes and reference electrode) were pressed against the textile.
Samples were absorbed to 300 µm holey formvar/carbon coated grids for ≈1 minute before a brief rinse in water, and negatively stained for 1 minute with 1% phosphotungstic acid.
The samples were absorbed by rehydration ZOOM strips (7 cm; pH 3-10 NL) overnight at room temperature and then focused under manufacturer's recommended conditions.
For refinement, apo-SWR1 and nucleosome-bound samples were absorbed onto glow-discharged Cu 200-mesh Quantifoil grids with a continuous-carbon support.
The samples were absorbed by rehydration ZOOM strips (7 cm; pH3-10NL) overnight at room temperature and then focused under manufacturer's recommended conditions.
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