Purifications were carried out using 1.8 volumes (i.e., 1.8 × the sample volume) of PEG Bead Solution and exactly as described in Steps 5 8 of Meyer et al. [ 12].
Flow rate of KIO3 solution, sample volume, carrier flow rate, and reaction coil were the selected factors for evaluation.
One quarter sample volume of 5× sucrose solution (1.65 M sucrose, 30 mM EDTA, 43 mM Tris, pH 7.5) was added to the homogenate and the sample mixed.
The impact of experimental variables namely pH, weight of sorbent, volume of 4% Triton X-114, eluting solution, and sample volume have been investigated and optimized in multivariate method using design Expert 7.0 software for statistical data analysis.
Performing an immunoassay inside a capillary requires only 200 ng tissue lysate present in 1 µL sample volume, 1 µL detection antibody solution and 1 µL of reporter molecule streptavidin-phycoerythrin.
[Term] id: MS:1000005 name: sample volume def: "Total volume of solution used".
is_a: MS:1000548 ! sample attribute relationship: has_units UO 0000098 ! milliliter [Term] id: MS:1000005 name: sample volume def: "Total volume of solution used".
KI-I2-solution (0.1 sample volume; 20 g/L KI (Sigma-Aldrich, Steinheim, Germany) and 2 g/L I2 (Merck) was added to the samples [ 23], and the colour development was visually determined (an exact photometric quantification was not possible because the undissolved particles in the suspension disturbed the measurements).
The variables of interest in the HS-HF-LPME were sample volume, extraction time, temperature of sample solution, ionic strength, stirring rate and dwelling time.
For the optimization phase, a centered face composite design 22 + star was employed to evaluate sample volume and flow rate of KIO3 solution, which were the factors identified in the screening phase as having more influence on the absorbance signal.
