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Additional advantages to the microbiocidal assay are its simplicity, short duration, small sample volume requirements, and that it requires only a minimal amount of specialized equipment to perform.
Ideally, new technologies will reduce sample volume requirements, lessen the amount of time required for microarray analysis (which now averages 24 hours), and reduce microarray costs to the extent that they can be used routinely in the clinic.
The sensor element design has certain advantages over existing SPR sensor configurations: (i) simplified fabrication; (ii) minimized sample volume requirements, and (iii) application to high throughput SPR analysis.
Lower sample volume requirements and reduced user intervention time compared to the manual instruments have improved turnover of calorimetry experiments in a high-throughput format where 25 or more runs can be conducted per day.
Bead-based multiplexed bioassays have many technical advantages, including near solution-phase kinetics, small sample volume requirements, many within-assay replicates to reduce measurement error, and, for some bead materials, the ability to synthesize analytes directly on beads via solid-phase synthesis.
Besides, the parallel plate geometry of the apparatus utilized here offers a distinctive edge over Couette type cells used for similar purposes as the latter is often not suitable for studies on well-entangled concentrated polymeric systems due to high viscosity and associated large sample volume requirements.
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The sample volume requirement is less than 150 μl and can be reduced significantly with optimized cartridge design.
An additional benefit of SVGs is the low sample volume requirement (typically ≤1 ml) to unveil the genomics of biologically relevant viruses, which is particularly advantageous for the investigation of the viral community in environments where it is technologically difficult or unfeasible, to collect large sample volumes.
The reported value for L corresponds to a sample volume requirement of 3 nL per assayed analyte in a 10 μm deep and 100 μm wide microfluidic channel, which is nearly 3 orders of magnitude smaller than that required on microarray based platforms.
The easy method of fabrication and the small sample volume requirement together with the high sensitivity towards uric acid measurement make this biosensor advantageous over the recently reported uric acid biosensors (Table 2).
Samples for T were assayed in duplicate DSL-40000), whereasampleses for E2 were determined in singleton (DS-43100), owing to the larger sample volume requirement of that assay.
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